Infinium FFPE DNA Restoration Solution

Archival formalin-fixed paraffin-embedded (FFPE) samples hold an abundance of invaluable information for human cancer studies. Because these samples generally yield highly degraded DNA, they perform poorly in most whole-genome genotyping studies. The Infinium HD FFPE DNA Restore Kit can repair these degraded DNA samples in preparation for use with the Infinium HD Assay. After DNA extraction using one of many commercially available kits, the Infinium HD FFPE QC Kit is used to evaluate the quality of prospective DNA samples to determine if they are usable. The kit provides primers for a real-time PCR assay that can be carried out using standard instrumentation available in most biological laboratories. Extracted FFPE samples that pass the QC test are then eligible for restoration using the Infinium HD  FFPE DNA Restore Kit.

Two 12-sample BeadChips, the HumanCytoSNP_FFPE-12 and HumanOmniExpress_FFPE, provide >250,000 and >600,000 genomic markers, respectively. The content on each array consists of intelligently selected tag SNPs that provide comprehensive coverage of the genome. With dense and even marker spacing, the BeadChip content can also be used to precisely detect structural aberrations, allowing researchers to accurately identify structural differences between paired-normal and tumor samples. The OmniExpress BeadChip provides coverage of 97% of the cancer genes identified by the Sanger Cancer Genome Project.