Sequencing - 200G to >20g
HiSeq 2500
Genome Analyzer IIx
MiSeq
Sequencing + Arrays
HiScanSQ
Arrays - Low- to High-Plex
HiScan
iScan
BeadXpress
Real-Time PCR System
Eco Real-Time
HumanHT-12 v4 Expression BeadChip Kits
| System: | iScan, BeadArray Reader, HiScanSQ |
|---|---|
| Technology: | BeadArray |
| Assay: | Direct Hybridization Assay |
| Applications: | Whole-Genome Gene Expression Analysis |
| Species: | Human |
| Contents: | Each kit includes 2 or 6 BeadChips plus reagents for hybridization, washing, and processing RNA samples |
The HumanHT-12 v4 Expression BeadChip provides high throughput processing of 12 samples per BeadChip without the need for expensive, specialized automation. The BeadChip is designed to support flexible usage across a wide-spectrum of experiments.
The updated content on the HumanHT-12 v4 Expression BeadChips provides more biologically meaningful results through genome-wide transcriptional coverage of well-characterized genes, gene candidates, and splice variants.
Each array on the HumanHT-12 v4 Expression BeadChip targets more than 31,000 annotated genes with more than 47,000 probes derived from the National Center for Biotechnology Information Reference Sequence (NCBI) RefSeq Release 38 (November 7, 2009) and other sources.
- Twelve-sample format enables large gene expression studies to be completed more quickly and economically
- HumanHT-12 Expression BeadChips use the Direct Hybridization Assay and are scanned on the BeadArray Reader, iScan system or HiScanSQ system.
- Sample labeling can be performed from as little as 50ng total RNA through a single round of IVT labeling with the TotalPrep RNA Labeling Kit (available directly from Ambion), which has been optimized for use with Illumina's Whole-Genome Expression Solutions.
Applications
Whole-Genome Gene Expression Analysis
The Direct Hybridization Assay offers the highest level of multiplexing for whole-genome expression profiling available. With the most up-to-date expression content and high-throughput processing, Illumina's whole-genome expression arrays produce high-quality data for large gene expression studies, efficiently and economically.
More...Documentation
- Whole-Genome Gene Expression Direct Hybridization Assay Guide
- Whole-Genome Gene Expression Direct Hybridization Assay Experienced User Card
Product Literature
RNA Analysis Solutions: Sample Labeling with TotalPrep from Ambion
The TotalPrep RNA Labeling Kit (available directly from Ambion) has been optimized for use with Illumina's Whole-Genome Expression Solutions. All reagents, including nucleotides, are included, making it even faster and easier to obtain ready-to-hybridize RNA. This streamlined protocol delivers outstanding quality with just a single round of IVT and input requirements of as little as 50 ng total RNA. The TotalPrep RNA Labeling Kit allows you to do more science at a lower cost when partnered with Illumina Gene Expression Solutions.
