DNA Activation Kits

DNA Activation Kits are used in the first steps of the GoldenGate protocol to make biotin-labeled DNA that is then purified for use in the GoldenGate Assay amplification step. The GoldenGate Assay allows for a high degree of loci multiplexing during the extension and amplification steps, minimizing time, reagent volumes, and material requirements of the process.

• The DNA sample used in this assay is activated for binding to paramagnetic particles
• This activation step is a highly robust process that requires a minimum input of DNA (250ng at 50ng/μl. Depending upon the multiplex level, this equates to only 160pg of DNA per SNP genotype call
• Assay oligonucleotides, hybridization buffer, and paramagnetic particles are combined with the activated DNA in the hybridization step