Canine SNP20 DNA Analysis Kits

The CanineSNP20 BeadChip presents a cost-effective and high-quality genotyping solution for researchers conducting genome-wide association studies within domestic dog breeds. The BeadChip was developed through Illumina's iSelect program in collaboration with researchers at the Broad Institute who completed the assembly of the canine genome, CanFam2.02, and presented the first comprehensive and high-quality reference sequence for the domestic dog. This BeadChip contains 22,362 validated single nucleotide polymorphism (SNP) probes derived from the CanFam2.0 assembly.

The SNP content featured on the CanineSNP20 BeadChip was selected from a diverse population of dog breeds. Single-direction reads from these breeds were aligned to a boxer reference sequence to identify polymorphic loci. Breed-specific SNPs were preferentially selected over the reference genome SNPs to provide researchers with a set of informative loci that are likely to segregate differently among breeds. This BeadChip presents an average of eight SNPs per megabase (Mb), providing ample SNP density for robust within-breed association studies.

Featuring highly polymorphic SNP content and providing uniform coverage, the CanineSNP20 BeadChip enables the interrogation of genetic variation in any domestic dog breed. This whole-genome genotyping microarray also empowers confident disease mapping. Additional CanineSNP20 BeadChip content includes 37 SNPs in 12 well-characterized cytokine genes for investigators interested in immunological studies.

The CanineSNP20 BeadChip is powered by the Infinium HD Assay that provides the industry's highest call rates, allows for flexible content deployment, and enables the detection and measurement of copy number variation.

• The assay's single-tube sample preparation without PCR or ligation steps significantly reduces labor and potential sample handling errors
• The BeadChip further reduces experimental variability by allowing researchers to interrogate up to 12 samples in parallel