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cBot Cluster生成系统

应用: DNA测序, 基因调控分析, 基于测序的转录组分析, SNP发现和结构变异分析, 细胞遗传分析, DNA-蛋白相互作用分析（ChIP-Seq）, 测序法甲基化分析, 小RNA发现和分析

cBot是Illumina测序中新一代的工作流程改进。cBot这个创新的自动化系统，能从单分子DNA模板中构建克隆集群，为Genome Analyzer的边合成边测序做准备。更多内容...

System Description

The cluster generation process on cBot has been significantly streamlined. Innovative features such as pre-packaged reagents, a single flow cell manifold, and integrated touch screen controls make the best next generation sequencing workflow even better.

cBot isothermally amplifies cDNA fragments that have been captured by complementary adapter oligonucleotides covalently bound to the surface of Illumina flow cells. Flow cells facilitate access of bound DNA to enzymes while ensuring high stability of surface-bound template and low non-specific binding of fluorescently-labeled nucleotides. Attached DNA fragments are extended and bridge amplified to create hundreds of millions of clusters, each of which contains ~1,000 identical copies of a single template molecule.